Molecular characterization of Leishmania infantum strains by kinetoplast DNA RFLP - PCR SHORT
نویسندگان
چکیده
Parole chiave Cane, Epidemiologia, kDNA RFLP-PCR, Leishmania infantum, Tipizzazione molecolare. Riassunto Il metodo più frequentemente utilizzato per la classificazione di Leishmania spp. è ancora rappresentato dalla caratterizzazione isoenzimatica sebbene sia un metodo laborioso e in alcuni casi non sufficientemente discriminante. Nel presente studio abbiamo utilizzato una reazione di amplificazione del DNA kinetoplastico seguita da digestione enzimatica, al fine di caratterizzare 15 ceppi di Leishmania infantum MON-1, isolati da altrettanti cani ospitati in canili pubblici di 7 differenti aree geografiche del nord Italia. Il ceppo di referenza MHOM/ TN/1980/IPT1 di L. infantum è stato incluso nello studio. A conferma del potere discriminante di questa metodica, sono stati osservati 6 differenti profili enzimatici, anche all’interno di uno stesso zimodema. A nostro avviso, kDNA RFLP-PCR può pertanto trovare un’applicazione utile per discriminare i casi di reinfezioni da quelli dovuti a ricadute, piuttosto che in studi epidemiologici su larga scala. Caratterizzazione molecolare di ceppi di Leishmania infantum mediante kDNA RFLP-PCR
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